Identification of key gene modules and pathways of human platelet transcriptome

Identification of key gene modules and pathways of human platelet transcriptome in acute myocardial infarction sufferers by way of co-expression community
Acute myocardial infarction (AMI) severely threatens human life. On this examine we aimed to systemically analyze the perform of key gene modules in human platelets in AMI. We used weighted gene co-expression community evaluation (WGCNA) to assemble a co-expression module, and analyzed the connection between potential modules and medical traits based mostly on platelet RNA-seq RPKM rely reads of 16 ST-segment elevation myocardial infarction (STEMI) sufferers and 16 non-STEMI (NSTEMI) sufferers offered by the GEO database.
Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) evaluation had been carried out with the DAVID tool. Hub genes had been calculated by the Cytohubba bundle. A complete of 3653 genes was chosen to assemble the co-expression modules.
A major correlation between BMI and the module with shade of sky-blue in STEMI. In NSTEMI, there was a major correlation between the sky blue module and CAD, the Salmon module and HT, and the Cyan module and HT. In STEMI, the Hub genes had been primarily enriched in capabilities associated to cell membrane sign transduction together with Aqp1, Armcx1, Gsta4, Hist3h2a and Il17re.
In NSTEMI, the Hub genes are associated primarily to vitality metabolism within the sky-blue module together with Olr1, Nap1l3, Gfer, Dohh, Crispld1 and Ccdc8b; they’re primarily associated to extracellular house and calcium binding within the Cyan module, together with Clec12b, Chd4, Asgr1, Armcx4, Chid1 and Alkbh7.
The hub genes within the Salmon module embody Ell3, Aldh1b1, Cavin4, Cabp4, Eif1ay and Dus3l. Our outcomes present a framework for co-expression gene modules in STEMI and NSTEMI sufferers, and determine key targets as biomarkers for sufferers with completely different subtypes of AMI.
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SRC kinase signaling inhibitor 1 (SRCIN1) Antibody

abx331175-100ul 100 ul
EUR 510

SRC kinase signaling inhibitor 1 (SRCIN1) Antibody

20-abx327632
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

HPGDS inhibitor 1

B1046-1 1 mg
EUR 217.2
Description: HPGDS inhibitor 1 is an oral potent and selective inhibitor of hematopoietic prostaglandin D synthase (HPGDS) with IC50 value of 0.7nM [1].Prostaglandin D2 (PGD2) is a mediator of allergy and inflammation.

Anti-Src Rabbit Monoclonal Antibody

M00107-1 100ug/vial
EUR 476.4
Description: Rabbit Monoclonal Src Antibody. Validated in IF, WB and tested in Human, Rat.

Human SRC kinase signaling inhibitor 1 (SRCIN1) ELISA Kit

abx385443-96tests 96 tests
EUR 1093.2

Rat SRC kinase signaling inhibitor 1 (SRCIN1) ELISA Kit

abx392007-96tests 96 tests
EUR 1093.2

Mouse SRC kinase signaling inhibitor 1 (SRCIN1) ELISA Kit

abx390644-96tests 96 tests
EUR 1093.2

Human SRC kinase signaling inhibitor 1, SRCIN1 ELISA KIT

ELI-13954h 96 Tests
EUR 988.8

Mouse SRC kinase signaling inhibitor 1, Srcin1 ELISA KIT

ELI-18126m 96 Tests
EUR 1038

pp60 c-src (521-533) (phosphorylated)

B5235-1 1 mg
EUR 319.2

SRC 1 Antibody

abx238217-100ug 100 ug
EUR 610.8

SRC Antibody

1-CSB-PA001803
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against SRC. Recognizes SRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IF:1/200-1/1000.ELISA:1/40000

SRC Antibody

1-CSB-PA001806
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against SRC. Recognizes SRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

SRC Antibody

1-CSB-PA001807
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against SRC. Recognizes SRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/10000

SRC Antibody

1-CSB-PA001809
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against SRC. Recognizes SRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/5000

SRC Antibody

1-CSB-PA080378
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against SRC. Recognizes SRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100

SRC Antibody

1-CSB-PA081645
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against SRC. Recognizes SRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:15-1:50

src Antibody

1-CSB-PA24479A0Rb
  • EUR 380.40
  • EUR 402.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against src. Recognizes src from Zebrafish. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:5000

SRC Antibody

1-CSB-PA050134
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against SRC. Recognizes SRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/5000

SRC Antibody

1-CSB-PA022650GA01HU
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against SRC. Recognizes SRC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

187-1, N-WASP inhibitor

B5251-1 1 mg
EUR 478.8

Srcin1 ELISA Kit| Mouse SRC kinase signaling inhibitor 1 ELISA

EF016287 96 Tests
EUR 826.8

YAP-TEAD Inhibitor 1 (Peptide 17)

A1149-1 1 mg
EUR 282

EGFR Inhibitor

C3327-1 1 mg
EUR 184.8
Description: EGFR inhibitor is a cell permeable, pyrimidine compound that selectively inhibits the EGFR kinase with IC50 value of 21 nM [1]. EGFR is a transmembrane protein, and is a receptor for members of epidermal growth factor family.

Chk2 Inhibitor

1702-1
EUR 216

SCD1 Inhibitor

1716-1
EUR 261.6

ACC2 Inhibitor

1717-1
EUR 261.6

Syk Inhibitor

1983-1
EUR 151.2

NFAT Inhibitor

A4539-1 1 mg
EUR 165.6
Description: Selective inhibitor of calcineurin-mediated dephosphorylation of nuclear factor of activated T cells (NFAT). Does not disrupt other calcineurin-dependent pathways. Inhibits NFAT activation and NFAT-dependent expression of endogenous cytokine genes in T cells.

Lck Inhibitor

A3539-1 1 mg
EUR 184.8
Description: Lck Inhibitor is a small-molecule inhibitor of with IC50 value of 7 nM [1].The lymphocyte specific kinase which expressed in NK cells and T-cells is a member of the Src kinase family.

Stat5 Inhibitor

9484-1
EUR 144

Human Proto-oncogene tyrosine-protein kinase Src (SRC)

1-CSB-EP022650HU
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Proto-oncogene tyrosine-protein kinase Src(SRC) expressed in E.coli

Human Proto-oncogene tyrosine-protein kinase Src (SRC)

1-CSB-EP022650HUe1
  • EUR 872.40
  • EUR 510.00
  • EUR 2331.60
  • EUR 1266.00
  • EUR 1602.00
  • EUR 615.60
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Proto-oncogene tyrosine-protein kinase Src(SRC) expressed in E.coli

Srcin1 ELISA Kit| Rat SRC kinase signaling inhibitor 1 ELISA Ki

EF019367 96 Tests
EUR 826.8

SRC - 1 (682–697)

5-01959 4 x 1mg Ask for price

SRC-1 (pT1179) Antibody

abx217070-100ug 100 ug
EUR 526.8

anti- SRC 1 antibody

FNab08217 100µg
EUR 658.5
Description: Antibody raised against SRC 1

T338C Src-IN-1

HY-16905 100mg
EUR 3531.6

Anti-SRC 1 antibody

PAab08217 100 ug
EUR 463.2

c-SRC (c-SRC) Antibody

20-abx007517
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

c-SRC (c-SRC) Antibody

20-abx008688
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

c-SRC (c-SRC) Antibody

abx232027-100ug 100 ug
EUR 661.2

c-SRC (c-SRC) Antibody

abx232028-100ug 100 ug
EUR 661.2

Src/ Rat Src ELISA Kit

ELI-18125r 96 Tests
EUR 1063.2

Phosphatase Inhibitor Cocktail 1 (100X in DMSO)

K1012-1 1 ml
EUR 122.4

NCOA1/SRC-1/KAT13A/ Rat NCOA1/ SRC- 1/ KAT13A ELISA Kit

ELA-E11292r 96 Tests
EUR 1063.2

EGFR/ErbB2 Inhibitor

C3285-1 1 mg
EUR 223.2
Description: IC50: 20 and 79 nM for EGFR and c-ErbB2, respectivelyEGFR/ErbB2 Inhibitor is an EGFR and c-ErbB2 inhibitor.EGFR and c-ErbB-2 are members of the epidermal growth factor receptor subfamily of protein tyrosine kinases.

Calpain Inhibitor XII

C5347-1 1 mg
EUR 174
Description: Ki: 19 nM for ?-calpain Calpain Inhibitor XII is a reversible and selective inhibitor of calpain I.Calpain is a protein belonging to the family of calcium-dependent, non-lysosomal cysteine proteases expressed ubiquitously in mammals and many other organisms.

MMP Inhibitor II

C4081-1 1 mg
EUR 142.8
Description: MMP Inhibitor II is a potent, reversible and broad-range inhibitor of matrix metalloproteinases (MMPs) with IC50 values of 24 nM, 18.4 nM, 30 nM and 2.7 nM for MMP-1, MMP-3, MMP-7 and MMP-9, respectively [1].

MMP-13 Inhibitor

C4331-1 1 mg
EUR 224.4
Description: IC50: 8 nMMMP-13 Inhibitor is a MMP-13 inhibitor.Matrix metalloproteinases (MMPs), a family of zinc endopeptidases, can degrade proteins of the extracellular matrix, such as collagens, elastins, matrix glycoproteins, and proteoclycans.

CFTR Inhibitor-172

2487-1
EUR 144

Bromodomain Inhibitor, (+)-JQ1

2070-1
EUR 196.8

Batimastat (MMP Inhibitor)

1704-1
EUR 216

Cathepsin G Inhibitor

1982-1
EUR 288

Smad3 Inhibitor, SIS3

2227-1
EUR 196.8

EZSolution? SCD1 Inhibitor

2548-1
EUR 248.4

FAS Inhibitor, C75

1547-1
EUR 216

MEK Inhibitor Set

A9907-1 1 Set
EUR 268.8

Bromodomain Inhibitor, (+)-JQ1

A1910-1 1 mg
EUR 129.6
Description: Bromodomain Inhibitor, (+)-JQ1 is a potent and highly specific inhibitor for the BET (bromodomain and extra-terminal) family of bromodomains. (+)-JQ1 binds to BRD4 bromodomains 1 and 2 with Kd values of ~ 50 and 90 nM, respectively.

USP7/USP47 Inhibitor

B2538-1
EUR 170.4

Cdk9 Inhibitor II

B2903-1
EUR 170.4

MLCK inhibitor peptide

B5236-1 1 mg
EUR 220.8

Rac1 Inhibitor W56

B5274-1 1 mg
EUR 478.8

Lyn peptide inhibitor

B5285-1 1 mg
EUR 721.2

DiscoveryProbe? Inhibitor Library

L1048-.1 100 uL/well(10 mM solution)
EUR 17264.4

Human Plasminogen Activator Inhibitor-1 (PAI-1) AssayMax ELISA Kit

EP1100-1 96 Well Plate
EUR 500.4

SERPINE1 Plasminogen Activator Inhibitor-1 Human Recombinant Protein

PROTP05121-1 Regular: 25ug
EUR 380.4
Description: SERPINE1 Human Recombinant fused to N-terminal His-Tag produced in E.Coli is a single, non-glycosylated polypeptide chain containing 400 amino acids (24-402) and having a molecular mass of 45 kDa.;The SERPINE1 is purified by proprietary chromatographic techniques.

Human Src kinase-associated phosphoprotein 1 (SKAP1)

1-CSB-EP769799HU
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Src kinase-associated phosphoprotein 1(SKAP1) expressed in E.coli

Plasminogen Activator Inhibitor 1 (PAI-1), hexapeptide, Human

SP-55196-1 0.5 mg
EUR 169.2

Human Proto-oncogene tyrosine-protein kinase Src(SRC) ELISA kit

1-CSB-EL022650HU
  • EUR 964.80
  • EUR 6118.80
  • EUR 3244.80
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Proto-oncogene tyrosine-protein kinase Src(SRC) in samples from serum, plasma, tissue homogenates, cell lysates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Src Antibody

AF4704 200ul
EUR 540

Src Antibody

AF6161 200ul
EUR 420

Src Antibody

AF6162 200ul
EUR 420

Src Antibody

AF6163 200ul
EUR 420

SRC siRNA

20-abx935129
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

SRC siRNA

20-abx935130
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

Src antibody

70R-37554 100 ug
EUR 327.6
Description: Rabbit Polyclonal Src antibody

Src antibody

70R-37603 100 ug
EUR 327.6
Description: Rabbit Polyclonal Src antibody

Src antibody

70R-34409 100 ug
EUR 392.4
Description: Rabbit polyclonal Src antibody

SRC antibody

70R-20514 50 ul
EUR 522
Description: Rabbit polyclonal SRC antibody

Src antibody

70R-32504 100 ug
EUR 392.4
Description: Rabbit polyclonal Src antibody

Src antibody

70R-32507 100 ug
EUR 392.4
Description: Rabbit polyclonal Src antibody

Src antibody

70R-32737 100 ug
EUR 392.4
Description: Rabbit polyclonal Src antibody

SRC Antibody

48186-100ul 100ul
EUR 399.6

SRC Antibody

48186-50ul 50ul
EUR 286.8

Src Antibody

48902-100ul 100ul
EUR 399.6

Src Antibody

48902-50ul 50ul
EUR 286.8

RR-SRC

5-01886 4 x 5mg Ask for price

Src Antibody

49317-100ul 100ul
EUR 399.6

Src Antibody

49317-50ul 50ul
EUR 286.8

Reproducibility and sensitivity of 36 strategies to quantify the SARS-CoV-2 genetic sign in uncooked wastewater: findings from an interlaboratory strategies analysis within the U.S

In response to COVID-19, the worldwide water group quickly developed strategies to quantify the SARS-CoV-2 genetic sign in untreated wastewater. Wastewater surveillance utilizing such strategies has the potential to enhance medical testing in assessing group well being. This interlaboratory evaluation evaluated the reproducibility and sensitivity of 36 commonplace working procedures (SOPs), divided into eight technique teams based mostly on pattern focus method and whether or not solids had been eliminated.
Two uncooked wastewater samples had been collected in August 2020, amended with a matrix spike (betacoronavirus OC43), and distributed to 32 laboratories throughout the U.S. Replicate samples analyzed in accordance with the mission’s high quality assurance plan confirmed excessive reproducibility throughout the 36 SOPs: 80% of the recovery-corrected outcomes fell inside a band of ±1.15 log10 genome copies per L with greater reproducibility noticed inside a single SOP (commonplace deviation of 0.13 log10).
The inclusion of a solids elimination step and the choice of a focus technique didn’t present a transparent, systematic impression on the recovery-corrected outcomes. Different methodological variations (e.g., pasteurization, primer set choice, and use of RT-qPCR or RT-dPCR platforms) typically resulted in small variations in comparison with different sources of variability.
These findings counsel that quite a lot of strategies are able to producing reproducible outcomes, although the identical SOP or laboratory ought to be chosen to trace SARS-CoV-2 developments at a given facility. The strategies confirmed a 7 log10 vary of restoration effectivity and restrict of detection highlighting the significance of restoration correction and the necessity to think about technique sensitivity when choosing strategies for wastewater surveillance.

ExTraMapper: Exon- and Transcript-level mappings for orthologous gene pairs

Motivation: Entry to large-scale genomics and transcriptomics knowledge from numerous tissues and cell traces allowed the invention of wide-spread various splicing occasions and various promoter utilization in mammalians. Between human and mouse, gene-level orthology is at present current for practically 16ok protein-coding genes spanning a various repertoire of over 200ok complete transcript isoforms.
Outcomes: Right here, we describe a novel technique, ExTraMapper, which leverages sequence conservation between exons of a pair of organisms and identifies a fine-scale orthology mapping on the exon after which transcript degree. ExTraMapper identifies greater than 350ok exon mappings, in addition to 30ok transcript mappings between human and mouse utilizing solely sequence and gene annotation data.
We reveal that ExTraMapper identifies a bigger variety of exon and transcript mappings in comparison with earlier strategies. Additional, it identifies exon fusions, splits, and losses because of splice web site mutations, and finds mappings between microexons which can be beforehand missed.
By reanalysis of RNA-seq knowledge from 13 matched human and mouse tissues, we present that ExTraMapper improves the correlation of transcript-specific expression ranges suggesting a extra correct mapping of human and mouse transcripts. We additionally utilized the tactic to detect conserved exon and transcript pairs between human and rhesus macaque genomes to spotlight the purpose that ExTraMapper is relevant to any pair of organisms which have orthologous gene pairs.
Availability: The supply code and the outcomes can be found

Unconventional viral gene expression mechanisms as therapeutic targets

In contrast to the human genome that includes principally noncoding and regulatory sequences, viruses have advanced underneath the constraints of sustaining a small genome measurement whereas increasing the effectivity of their coding and regulatory sequences.
Because of this, viruses use methods of transcription and translation wherein a number of of the steps within the standard gene-protein manufacturing line are altered. These various methods of viral gene expression (also called gene recoding) will be uniquely led to by devoted viral enzymes or by co-opting host components (referred to as host dependencies).
Focusing on these distinctive enzymatic actions and host components exposes vulnerabilities of a virus and gives a paradigm for the design of novel antiviral therapies. On this Assessment, we describe the categories and mechanisms of unconventional gene and protein expression in viruses, and supply a perspective on how future primary mechanistic work might inform translational efforts which can be aimed toward viral eradication.

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